![]() The persistence and emergence of new multi-drug resistant Mycobacterium tuberculosis (M. Ion Torrent sequencing may facilitate tracking and monitoring geographically diverse multidrug-resistantĪnd extensively drug-resistant strains and could potentially be integrated into selected regional and reference settings throughout ![]() Importantly, previously uncharacterized mutations in rpoB (V194I), rrs (G878A), and pncA (Q122Stop) genes were observed. In several rifampin-resistant isolates, Ion Torrent sequencing revealed uncommon substitutions (H526R andĭ516G) that did not have a defined mutation by LPA. ![]() Ion Torrent sequencing exhibited 100% (26/26) concordance to phenotypic resistance obtainedīy MGIT 960 culture and genotypic rpoB and katG results by LPA. A novel protocol for full-length Mycobacterium tuberculosis gene analysis of first- and second-line drug resistance was developed using the Ion Torrent Personal Genome Machine (PGM).įive genes-rpoB (rifampin), katG (isoniazid), pncA (pyrazinamide), gyrA (ofloxacin/fluoroquinolone), and rrs (aminoglycosides)-were amplified and sequenced, and results were compared to those obtained by genotypic Hain line probeĪssay (LPA) and phenotypic Bactec MGIT 960 analysis using 26 geographically diverse South African clinical isolates collectedīetween July and November 2011.
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